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  5. Invitrogen™ Human Apolipoprotein C3 ELISA Kit

Invitrogen™ Human Apolipoprotein C3 ELISA Kit

Catalog No. EHAPOC3
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96 Tests
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Catalog No. Quantity
EHAPOC3 96 Tests
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Catalog No. EHAPOC3 Supplier Invitrogen™ Supplier No. EHAPOC3

Description

ELISA

The Human Apolipoprotein C3 (Hu ApoC3) ELISA quantitates Hu ApoC3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoC3. Principle of the method The Human ApoC3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apolipoprotein C-III, also known as apo-CIII, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.

Specifications

Accession Number P02656
Assay Range 2.44-10000 pg/mL
Assay Sensitivity 2.5 pg/mL
Calibration Range 2.5 pg/mL to 10,000 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family GPCR (G-Protein Coupled Receptors)
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17 E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Apoc3; APOCIII; Apo-CIII; apoC-III; Apolipoprotein; Apolipoprotein C3; apolipoprotein C-3; apolipoprotein C-III; HALP2; MGC150353Apoc3; APOCIII; Apo-CIII; apoC-III; Apolipoprotein; Apolipoprotein C3; apolipoprotein C-3; apolipoprotein C-III; HALP2; MGC150353; APOCIII; HALP2
Gene ID (Entrez) 345
Gene Symbol APOC3
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases Apo-CIII, ApoC-III, Apolipoprotein C3
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Cardiovascular Research
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym APOCIII, HALP2
Target ApoC3
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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