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NEFM Rabbit anti-Bovine, Chicken, Equine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Pierce Biotechnology Inc PA110015

Catalog No. 01-670-272



Description

Description

In Western blot, this antibody detects bands at ∽145kDa in crude homogenates of rat cerebellum homogenate. Human and bovine run slower at ∽160kDa. A minor band at1∽110kDa is also frequently seen, which appears to be an in-vivo proteolytic fragment of NF-M.

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Specifications

Specifications

NEFM
Polyclonal
whole serum with 5mM sodium azide
O77788, P16053, P07197, P08553, P08552, P12839
NFM, NF-M, NMC,160 kD neurofilament protein, neurofilament medium polypeptide, neurofilament 3, NEF3
Rabbit
IgG
RUO
Antibody
Polyclonal
Bovine, Chicken, Equine, Human, Mouse, Porcine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Unconjugated
NEFM
Liquid
NEFM
Recombit fusion protein containing the extreme C-terminal segment of rat NF-M
100 μL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
100054129, 100625407, 18040, 24588, 281347, 396206, 4741
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