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anti-Myelin Basic Protein Polyclonal, Thermo Scientific™


Myelin Basic Protein Polyclonal Ab PA1-10008 from Thermo Scientific™ detects Myelin Basic Protein in Bv, Hu, Ms and Rt samples, and is validated for ICC, IF, IHC and WB

Manufacturer: pierce biotechnology inc  PA110008

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Catalog No. 01-670-265

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Description & Specifications

Specifications

Formulation 100μL purified antibody in PBS and 10mM sodium azide
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Western Blot
Gene Accession No. P02686, P02687, P02688, P04370, P15720, P81558, P83487
Storage Requirements avoiding freeze/thaw cycles.
Gene Alias MGC99675, MBP
Gene Symbols MBP
Species Reactivity Bovine, Human, Mouse, Rat
Gene ID (Entrez) 100063306, 17196, 24547, 396217, 414286, 4155, 618684
Immunogen Full length purified myelin basic protein isolated from cow nerve
Quantity 100μL
Cross Reactivity Bovine
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Format Purified
Host Species Chicken
Conjugate Unlabeled
Primary or Secondary Primary
Monoclonal or Polyclonal Polyclonal
Antigen Myelin Basic Protein
Isotype IgY

Myelin Basic Protein Polyclonal Ab PA1-10008 from Thermo Scientific™ detects Myelin Basic Protein in Bv, Hu, Ms and Rt samples, and is validated for ICC, IF, IHC and WB

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are three different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5 and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typical show two bands on Western blots, one at about 22kDa and another at about 18 kDa.
Host Species: Chicken
Cross Reactivity: Bovine, Human, Mouse, Rat
Isotype: IgY
Immunogen: Full length purified myelin basic protein isolated from cow nerve
Formulation: 100μL purified antibody in PBS and 10mM sodium azide
Synonyms: MGC99675, MBP
Uniprot Accession: P02686, P02687, P02688, P04370, P15720, P81558, P83487
Entrez GeneID: 100063306, 17196, 24547, 396217, 414286, 4155, 618684
Gene Symbol: MBP
In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ∽21.5kDa, 18.5kDa, 17kDa and 14kDa.
References:
Test: ICC, Dilution: 1:50, Species: Rt, Reference: Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels. (Oct 2014) Dev. Growth Differ., Vol: 56, 8, 583-94, Teh DB,Ishizuka T,Yawo H; Pubmed ID: 25283796