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MBP Chicken anti-Bovine, Human, Mouse, Porcine, Rat, Polyclonal, Invitrogen™

Chicken Polyclonal Antibody

Manufacturer:  Pierce Biotechnology Inc PA110008

Catalog No. 01-670-265



Description

Description

In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ∽21.5kDa, 18.5kDa, 17kDa and 14kDa. | Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are three different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5 and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typical show two bands on Western blots, one at about 22kDa and another at about 18 kDa.
Specifications

Specifications

MBP
Polyclonal
PBS with 0.02% sodium azide
P02687, P15720, P83487, P02686, P04370, P81558, P02688
MGC99675, MBP
Chicken
IgY
100 μL
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
100063306, 17196, 24547, 396217, 414286, 4155, 618684
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Unconjugated
MBP
Liquid
MBP
Full length purified myelin basic protein isolated from cow nerve
PEG precipitation
RUO
Antibody
Polyclonal
Bovine, Human, Mouse, Porcine, Rat
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