Myelin Basic Protein Polyclonal Antibody, Invitrogen™

Description

Myelin Basic Protein Antibody, PA1-10008, from Invitrogen. Species Reactivity: Bovine, Human, Mouse, Rat; Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot.

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are three different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5 and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typical show two bands on Western blots, one at about 22kDa and another at about 18 kDa.
Host Species: Chicken
Cross Reactivity: Bovine, Human, Mouse, Rat
Isotype: IgY
Immunogen: Full length purified myelin basic protein isolated from cow nerve
Formulation: 100μL purified antibody in PBS and 10mM sodium azide
Synonyms: MGC99675, MBP
Uniprot Accession: P02686, P02687, P02688, P04370, P15720, P81558, P83487
Entrez GeneID: 100063306, 17196, 24547, 396217, 414286, 4155, 618684
Gene Symbol: MBP
In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ∽21.5kDa, 18.5kDa, 17kDa and 14kDa.
References:
Test: ICC, Dilution: 1:50, Species: Rt, Reference: Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels. (Oct 2014) Dev. Growth Differ., Vol: 56, 8, 583-94, Teh DB,Ishizuka T,Yawo H; Pubmed ID: 25283796

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