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Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction

Supplier:  Cytiva RPN3001

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Catalog No. 45-000-923




    • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
    • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
    • No need to strip blots before reprobing.
    • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
    • Consistent results combining strong signals with very low backgrounds.





20 min.
ECL Direct Labeling and Detection System
Colony or plaque screens, dot blots and PCR product analyses
For Labeling 10μg Nucleic Acid
DNA labeling reagent (2 x 0.5mL); Glutaraldehyde solution (2 x 0.5mL); Water (2 x 1.0mL); l Hind III control DNA 10μg/mL (2 x 0.1mL); Gold hybridization buffer (2 x 500mL); Blocking agent (2 x 25g); ECL detection reagent 1 (2 x 125mL); ECL detection reagent 2 (2 x 125mL)
2°C to 8°C


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