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Cytiva Amersham™ ECF™ Substrate for Western Blotting

Used for detection of protein blots with fluorescence scanning equipment.  Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.

Manufacturer:  Cytiva RPN5785

Catalog No. 45-000-947



Includes: ECF Substrate, 60mL; Dilution buffer

Description

Description

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

  • For use with fluorescence scanning equipment
  • Sensitivity comparable to chemiluminescence
  • Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminate need for darkroom and film

Western blotting, dot, and slot blotting

Specifications

Specifications

Sufficient reagents for detecting 2500cm2 membrane
ECF Substrate, 60mL, Dilution Buffer
Fluorescence scanning equipment
SDS
Documents

Documents

Product Certifications
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