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Cytiva Amersham™ ECF™ Substrate for Western Blotting

Catalog No. 45000947
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Sufficient reagents for detecting 2500cm2 membrane
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Catalog No. Quantity
45000947 Sufficient reagents for detecting 2500cm2 membrane
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Catalog No. 45000947 Supplier Cytiva Supplier No. RPN5785

Description

Includes

ECF Substrate, 60mL; Dilution buffer

Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

  • For use with fluorescence scanning equipment
  • Sensitivity comparable to chemiluminescence
  • Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminate need for darkroom and film

Western blotting, dot, and slot blotting

Specifications

For Use With (Application) Fluorescence scanning equipment
Includes ECF Substrate, 60mL, Dilution Buffer
Quantity Sufficient reagents for detecting 2500cm2 membrane