The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast.
Supplier: Invitrogen™ 12183555
The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.
Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 ÎĽg column-binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.
Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
Not high-throughput compatible (Manual)
Shipping Condition: Room temperature
| • 100 mL TRIzol Reagent; room temperature • 1 PureLink RNA Mini Kit: 125 mL Lysis Buffer; room temperature • 50 mL Wash Buffer I; room temperature • 15 mL Wash Buffer II; room temperature • 15.5 mL RNase-free Water; room temperature • 50 PureLink Spin Cartridges with Collection Tubes; room temperature • 50 Collection Tubes; room temperature • 50 Recovery Tubes; room temperature |
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| 1 hr. | |
| Bacteria, Cells, Plant Samples, Tissue, Yeast | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Room Temperature | |
| Up to 350 ÎĽg |
| Silica Spin Column, Organic Extraction | |
| 30 to 300 ÎĽL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling | |
| Mini | |
| Bacteria: ≤107 Cells: ≤107 Plant: ≤107, ≤100 mg Tissue: ≤100 mg Yeast: ≤107 |
For Research Use Only. Not for use in diagnostic procedures.
