The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
Supplier: Invitrogen™ AM1931
The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
| • 50 Filter Cartridges with Collection Tubes; store at room temperature • 50 Elution Tubes (1.5 mL); room temperature • 15 mL Wash Solution 1 (concentrated); room temperature • 28 mL Wash Solution 2/3 (concentrated); 4°C • 25 mL Lysis Solution; 4°C • 200 μL LCM Additive; -20°C • 55 μL DNase; -20°C • 600 μL 10X DNase I Buffer; -20°C • 150 μL DNase Inactivation Reagent; -20°C • 5 mL Elution Solution; room temperature |
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| 15 min. (After sample homogenization) | |
| Cells, Tissue, Laser Capture Microdissection (LCM) | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Cells: ≤105 Tissue: ≤10 mg |
| Silica Spin Column | |
| 10–20 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.
