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Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit
Description
Includes
50 Micro Filter Cartridge Assembly; 50 Micro Elution Tubes; 15mL Wash Solution 1 Concentrate; 28mL Wash Solution 2/3 concentrate; 25 Lysis Solution; 200μL LCM Additive; 55μL DNase 2U/μL; 600μL 10X DNase I Buffer; 150μL DNase Inactivation Reagent; 5mL Elution Solution
The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
Specifications
Specifications
| Content And Storage | • 50 Filter Cartridges with Collection Tubes; store at room temperature • 50 Elution Tubes (1.5 mL); room temperature • 15 mL Wash Solution 1 (concentrated); room temperature • 28 mL Wash Solution 2/3 (concentrated); 4°C • 25 mL Lysis Solution; 4°C • 200 μL LCM Additive; -20°C • 55 μL DNase; -20°C • 600 μL 10X DNase I Buffer; -20°C • 150 μL DNase Inactivation Reagent; -20°C • 5 mL Elution Solution; room temperature |
| Isolation Technology | Silica Spin Column |
| Purification Time | 15 min. (After sample homogenization) |
| Elution Volume | 10–20 μL |
| Sample Type | Cells, Tissue, Laser Capture Microdissection (LCM) |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 50 Preps |
| Shipping Condition | Box 1: Dry Ice Box 2: Room Temperature |
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Frequently Asked Questions (FAQs)
RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).
For Research Use Only. Not for use in diagnostic procedures.