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Invitrogen™ RNAqueous™-96 Automated Kit
Description
Includes
4 Filter Plates, 4 Collection Plate with lids, 4 Culture Plates, 1 Heat Plate with lid, 100mL Nuclease-free Water, 100mL Elution Solution, 100mL Lysis/Binding Solution, 100mL Rebinding Mix, and 2 x 175mL Wash Solution Concentrate, (1.25mL DNase I (20U/μL) and 12mL DNase 1 Buffer
The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications.
Specifications
Specifications
| Content And Storage | • 4 Filter Plates; store at room temperature • 4 Collection Plates with lids; room temperature • 4 Culture Plates; room temperature • 1 Heat Plate with Lid; room temperature • 100 mL Nuclease-free Water; room temperature • 100 mL Elution Solution; room temperature • 100 mL Lysis/Binding Solution; 4°C • 100 mL Rebinding Mix; 4°C • 2 x 175 mL Wash Solution (concentrated); 4°C • 1.25 mL DNase 1; -20°C • 12 mL DNase 1 Buffer; -20°C |
| Isolation Technology | Vacuum Filter Plate (Glass Fiber Filter) |
| Elution Volume | 105 μL |
| Sample Type | Cells, Tissue |
| Final Product Type | Total RNA |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Equipment) | MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 384 Preps |
| Shipping Condition | Box 1: Dry Ice Box 2: Room Temperature |
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Frequently Asked Questions (FAQs)
RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).
For Research Use Only. Not for use in diagnostic procedures.