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Invitrogen™ RNAqueous™-96 Automated Kit
The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
Supplier: Invitrogen™ AM1812
Includes: 4 Filter Plates, 4 Collection Plate with lids, 4 Culture Plates, 1 Heat Plate with lid, 100mL Nuclease-free Water, 100mL Elution Solution, 100mL Lysis/Binding Solution, 100mL Rebinding Mix, and 2 x 175mL Wash Solution Concentrate, (1.25mL DNase I (20U/μL) and 12mL DNase 1 Buffer
Description
The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications.
Specifications
| • 4 Filter Plates; store at room temperature • 4 Collection Plates with lids; room temperature • 4 Culture Plates; room temperature • 1 Heat Plate with Lid; room temperature • 100 mL Nuclease-free Water; room temperature • 100 mL Elution Solution; room temperature • 100 mL Lysis/Binding Solution; 4°C • 100 mL Rebinding Mix; 4°C • 2 x 175 mL Wash Solution (concentrated); 4°C • 1.25 mL DNase 1; -20°C • 12 mL DNase 1 Buffer; -20°C |
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| 105 μL | |
| Total RNA | |
| MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems | |
| 384 Preps | |
| Cells: ≤105 Tissue: 0.1–1 mg |
| Vacuum Filter Plate (Glass Fiber Filter) | |
| Cells, Tissue | |
| High-throughput Compatible | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.