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Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit
Description
Includes
600μL 10X DNase I Buffer; 45μL DNase I 2U/μL; 1mL Formaldehyde Load Dye; 100μL Linear Acrylamide 5mg/mL; 50μL Positive Control Primers; 1mL 5M Ammonium Acetate; 35mL Water for 64% Ethanol; 600mL DNase Inactivation Reagent; 30mL Lysis/Binding Solution; 25mL Wash Solution 1; 35mL Wash Solution 2/3; 3 Pestles for tissue disruption; 30 Filter Cartridges; 60 Collection Tubes; 25mL Elution Solution
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
- DNA-free RNA for RT-PCR
- Rapid, phenol-free total RNA isolation
- Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous-4PCR Kit
The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
Specifications
Specifications
| Content And Storage | • 600 μL 10X DNase 1 Buffer; store at -20°C • 45 μL DNase 1; -20°C • 1 mL Formaldehye Load Dye; -20°C • 100 μL Linear Acrylamide; -20°C • 50 μL Positive Control Primers; -20°C • 1 mL 5M Ammonium Acetate; -20°C • 35 mL Water; 4°C • 600 μL DNase Inactivation Reagent; 4°C • 30 mL Lysis/Binding Solution; 4°C • 25 mL Wash Solution #1; 4°C • 35 mL Wash Solution 2/3 (concentrated); 4°C • 3 Pestles; room temperature • 30 Filter Cartridges; room temperature • 60 Collection Tubes; room temperature • 25 mL Elution Solution; room temperature |
| Isolation Technology | Silica Spin Column |
| Purification Time | 10 min. (After sample homogenization) |
| Elution Volume | 50–120 μL |
| Sample Type | Tissue, Cells |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 30 Preps |
| Shipping Condition | Box 1: Dry Ice, Box 2: Room Temperature |
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Frequently Asked Questions (FAQs)
RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).
For Research Use Only. Not for use in diagnostic procedures.