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Invitrogen™ RiboMinus™ Transcriptome Isolation Kit, bacteria
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample.
Supplier: Invitrogen™ K155004
Comprend: The RiboMinus Isolation Kits include RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.
Description
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95 to 98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA. This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis, library construction, and qRT-PCR.
• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios
• Not high-throughput compatible (Manual)
Spécifications
| The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at 4°C, and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored. |
|
| 1.25 hr. | |
| Transcriptome RNA | |
| Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction | |
| 12 preps | |
| Transcriptome RNA | |
| 1 μg |
| Magnetic Bead | |
| Total RNA (Bacterial) | |
| Not High-throughput Compatible (Manual) | |
| RiboMinus | |
| Up to 10 μg total RNA | |
| RiboMinus | |
| Magnetic Bead |
For Research Use Only. Not for use in diagnostic procedures.