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Invitrogen™ RiboMinus™ Human/Mouse Transcriptome Isolation Kit
The RiboMinus™ Human/Mouse Transcriptome Isolation Kit constitutes a novel purification system that enriches the whole spectrum of mRNA species by depleting large ribosomal RNA molecules in a total RNA sample.
Supplier: Invitrogen™ K155001
Comprend: Contains two modules; the RiboMinus Human/Mouse Probe Module and the Concentration Module. The RiboMinus Human/Mouse Probe Module contains the RiboMinus magnetic beads, the RiboMinus human/mouse probe, and binding buffer. The Concentration Module contains spin cartridges, wash tubes, recovery tubes, binding buffer, wash buffer, and RNase-free water.
Description
The RiboMinus™ Human/Mouse Transcriptome Isolation Kit constitutes a novel purification system that enriches the whole spectrum of mRNA species by depleting large ribosomal RNA molecules in a total RNA sample. The kit removes >95% of 18S and 28S rRNA molecules from human or mouse total RNA without relying on current polyA+ purification methods. This increases the representation of mRNA species, including non-polyA-tailed, fragmented mRNA or other regulatory RNAs that cannot be purified via polyA-selection methods. Purified RNA is suitable for several downstream applications, including, library construction, RT-PCR, and microarray.
Not high-throughput compatible (Manual)
Spécifications
| The RiboMinus™ Human/Mouse Transcriptome Isolation Kit contains two modules: the RiboMinus™ Human/Mouse Probe Module and the Concentration Module The RiboMinus™ Human/Mouse Probe Module contains the RiboMinus™ magnetic beads, the RiboMinus™ human/mouse probe, and binding buffer. Store at 4°C The Concentration Module contains spin cartridges, wash tubes, recovery tubes, binding buffer, wash buffer, and RNase-free water. Store at room temperature. All reagents are guaranteed stable for 6 months when properly stored. |
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| Total RNA | |
| Not High-throughput Compatible (Manual) | |
| RiboMinus | |
| Transcriptome RNA | |
| Magnetic Bead |
| Magnetic Bead | |
| Transcriptome RNA | |
| Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction | |
| 6 preps | |
| RiboMinus |
For Research Use Only. Not for use in diagnostic procedures.