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Invitrogen™ PureLink™ Total RNA Blood Kit 
PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
Supplier: Invitrogen™ K156001
Description
PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
Simple RNA Purification from Whole Blood
PureLink Total RNA Blood Kit is designed for purifying from 50 to 500 μL fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.
Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink Total RNA Blood Kit.
- RNA purification from whole blood in under an hour
- Yields high-quality RNA devoid of non-nucleated erythrocytes
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
- Northern Blotting Kits & Reagents
- Nuclease protection assays
- Microarray
- cDNA synthesis
- Real-Time PCR (qPCR)
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood
Order Info
Shipping Condition: Room temperature
Specifications
| • 17.5 mL Lysis Buffer L3; store at room temperature • 175 mL Lysis Buffer L5; room temperature • 60 mL Wash Buffer W4; room temperature • 10 mL Wash Buffer W5; room temperature • 5 mL RNase-free Water; room temperature • 50 Spin Cartridges with Collection Tubes; room temperature • 50 Wash Tubes (2 mL); room temperature • 50 Elution Tubes (1.7 mL); room temperature |
|
| 20 min. (After sample homogenization) | |
| Blood | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Room Temperature | |
| 1000 μg (Binding capacity) |
| Silica Spin Column | |
| 30–100 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Mini | |
| Blood: ≤500 μL |
For Research Use Only. Not for use in diagnostic procedures.