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Invitrogen™ PureLink™ FFPE RNA Isolation Kit
Description
Includes
Sufficient for up to 50 reactions and each reaction is capable of purifying total RNA for up to 10 slices of 10 μm-thick FFPE sections. Kit includes: Deparaffinization/Protinase K digestion buffer, Protein/RNase Degrading buffer, 50 spin columns, 50 wash tubes, 50 recovery tubes, Binding buffer, and Wash buffer.
The PureLink FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization.
Rapid and Non-Toxic Isolation Procedure
The PureLink FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 to 40 minutes instead of days, as is required for older FFPE isolation methods. The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.
High Yield, High Quality RNA
The PureLink FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length. In addition, the PureLink FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.
Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and Products
RNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.
- Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens
- Isolates RNA with maximum yield and minimum degradation
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from FFPE Samples
Specifications
Specifications
| Content And Storage | • 15 mL Melting Buffer; store at room temperature • 1 mL Proteinase K in buffer; room temperature • 20 mL Binding Buffer L3; room temperature • 20 mL Wash Buffer W5; room temperature • 4 mL RNase-free Water; room temperature • 50 Spin Cartridges with Collection Tubes; room temperature • 50 Wash Tubes; room temperature • 50 Recovery Tubes; room temperature • 0.1 mL DNase I, Amplification Grade; -20°C |
| Isolation Technology | Silica Spin Column |
| Elution Volume | 50 μL |
| Sample Type | Formalin-fixed, Paraffin-embedded (FFPE) Tissue |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 50 Preps |
| Scale | Mini |
| Shipping Condition | Box 1: Ice Box 2: Room Temperature |
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Frequently Asked Questions (FAQs)
Since the quality of RNA is dependent on the quality of FFPE tissues itself, the amplifiable cDNA length is variable. Competitors' kits have been shown to max out around 400 bp, while in our experience, with our patent-pending method in the PureLink FFPE RNA Isolation Kit, most RT-PCR products are about 400 bp to 800 bp, and can even be over 1.3 kb.
The PureLink FFPE RNA Isolation Kit provides a simple and rapid method for isolating total RNA from formalin-fixed paraffin-embedded (FFPE) samples with high-quality results. A fast and easy heating procedure replaces traditional labor-intensive protocols that use toxic organic solvents for deparaffinization and give disappointing results. This reduces the time required for total RNA isolation to 30-40 minutes instead of the hours or a full day required by traditional FFPE isolation methods. In addition, the RNA isolated with the PureLink FFPE RNA Isolation Kit is of higher quality and demonstrates increased amplifiable RNA size, allowing for RT-PCR products that are longer than those obtained with competitor kits and reagents. Isolated RNA is ideal for use in all gene expression studies, including microarrays, RT-PCR, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.