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Invitrogen™ PureLink™ FFPE RNA Isolation Kit 
The PureLink FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens.
Supplier: Invitrogen™ K156002
Includes: Sufficient for up to 50 reactions and each reaction is capable of purifying total RNA for up to 10 slices of 10 μm-thick FFPE sections. Kit includes: Deparaffinization/Protinase K digestion buffer, Protein/RNase Degrading buffer, 50 spin columns, 50 wash tubes, 50 recovery tubes, Binding buffer, and Wash buffer.
Description
The PureLink FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization.
Rapid and Non-Toxic Isolation Procedure
The PureLink FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 to 40 minutes instead of days, as is required for older FFPE isolation methods. The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.
High Yield, High Quality RNA
The PureLink FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length. In addition, the PureLink FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.
Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and Products
RNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.
- Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens
- Isolates RNA with maximum yield and minimum degradation
Not high-throughput compatible (Manual)
Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from FFPE Samples
Specifications
| • 15 mL Melting Buffer; store at room temperature • 1 mL Proteinase K in buffer; room temperature • 20 mL Binding Buffer L3; room temperature • 20 mL Wash Buffer W5; room temperature • 4 mL RNase-free Water; room temperature • 50 Spin Cartridges with Collection Tubes; room temperature • 50 Wash Tubes; room temperature • 50 Recovery Tubes; room temperature • 0.1 mL DNase I, Amplification Grade; -20°C |
|
| 50 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Mini | |
| 3–8 pieces of FFPE with 10 μm thick sections |
| Silica Spin Column | |
| Formalin-fixed, Paraffin-embedded (FFPE) Tissue | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Box 1: Ice Box 2: Room Temperature |
|
| >1 μg |
For Research Use Only. Not for use in diagnostic procedures.