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Invitrogen™ PureLink™ DNase Set

Catalog No. 12185010
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Catalog No. 12185010 Supplier Invitrogen™ Supplier No. 12185010
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Description

Includes

PureLink™ DNase (1500U, lyophilized), 10X DNase I Reaction Buffer (1mL), RNase-Free Water (1mL)

The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink™ protocols.

Using the PureLink™ DNase Set
The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink™ protocols. The degree of DNA digestion performed by PureLink™ DNase is optional for most PureLink™ RNA kits, due to the efficient removal of the majority of DNA by the PureLink™ technology; however, some downstream applications that are sensitive to small amounts of DNA may require further removal of DNA.

  • RNase-free for use in RNA purification
  • Optimized for on-column digestion
  • Lyophilized for stability

Order Info

Shipping Condition: Room Temperature

Specifications

Concentration 10X
Content And Storage Contains:
• PureLink™ DNase (1500 U, lyophilized)
• 10X DNase I Reaction Buffer (1 mL)
• RNase-Free Water (1 mL)

Store at 4°C upon receipt. After reconstituted, PureLink™ DNase should be stored at -20°C.
No. of Reactions 50 Preps
Quantity 50 Preps
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

How can I inactivate DNase I?

Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 molar ratio of Mg++ ions:EDTA) to chelate the Mg++ ions in the DNase I buffer. Heat for 10 min at 65 degrees C.

Note: It is vital that the EDTA be at least at 2 mM prior to heat-inactivation to avoid Mg-dependent RNA hydrolysis.

DNA-free and Turbo-free versions of DNase I can be inactivated with included DNase Inactivation Reagent.
Are your DNase I products RNase-free?

Most of our DNase I products are guaranteed free of RNase activity. However, please note that Cat. No. 18047-019 is not tested for RNAse and is recommended primarily for protein applications. The other products are suitable for removing DNA from both RNA and protein preparations, for nick translating DNA, and for generating random fragments of DNA. For more demanding RT-PCR applications, we recommend using DNAse I, Amplification Grade.

For Research Use Only. Not for use in diagnostic procedures.