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Invitrogen™ Poly(A) Tailing Kit

Catalog No. AM1350
Encompass
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25 Reactions
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Catalog No. Quantity
AM1350 25 Reactions
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Catalog No. AM1350 Supplier Invitrogen™ Supplier No. AM1350
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Description

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions.

  • Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
  • Enhances translational efficiency in vivo
  • Reaction can be adjusted to control for tail length
  • Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit

Specifications

For Use With (Application) miRNA analysis
Product Line Ambion
Quantity 25 Reactions
Includes E-PAP, 5X E-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at -20°C.

Nuclease-free Water may be stored at any temperature.

Frequently Asked Questions (FAQs)

What is the maximum input amount of RNA that can be used per reaction when working with the Poly(A) Tailing Kit (Cat. No. AM1350)?

The maximum recommended input is 40 µg of RNA. This RNA could be total RNA, MEGAscript transcript or even bacterial RNA.

For Research Use Only. Not for use in diagnostic procedures.