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Invitrogen™ Plant RNA Isolation Aid

Catalog No. AM9690
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10 mL
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AM9690 10 mL
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Catalog No. AM9690 Supplier Invitrogen™ Supplier No. AM9690
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Description

Recommended for use with the RNAqueous Kit for purification of total RNA from plant tissues. One bottle of 10 mL Invitrogen reagent is provided.

Recommended for use with the RNAqueous Kit for purification of total RNA from plant tissues. One bottle of 10 mL Invitrogen reagent is provided.

Invitrogen's Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in plant tissues. Glass fiber filter–based methods of isolation, such as the RNAqueous Kit, are especially suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation. When used with the RNAqueous Kit, the procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added. A brief centrifugation then removes the contaminants. The supernatant is passed through a glass fiber filter which binds RNA. The filter is then washed and the RNA is eluted in a small volume (50μL or more) of elution solution.

• Removes common plant contaminants such as polyphenolics and polysaccharides
• No phenol extraction, alcohol precipitation, or proteinase digestion steps required when used with the RNAqueous Kit
• Not high-throughput compatible (Manual)

Accessory Product
The Plant RNA Isolation Aid is designed for use with the RNAqueous Kit (Cat. No. AM1912) for RNA purification from plant tissues.

Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Plant Cells

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store Plant RNA Isolation Aid at room temperature or at 4°C.
Isolation Technology PVP Reagent
Sample Type Plant Samples
Final Product Type RNA Supernatant (for use with RNAqueous™ kit)
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Equipment) RNAqueous™ Kit
Quantity 10 mL
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

I used the Plant RNA Isolation Aid along with the RNAqueous kit to isolate total RNA from my plant sample. My RNA preparation is too dilute. What do you recommend?

Yields may be improved in some cases by keeping the preparation more concentrated, especially if the tissue has a high water content. Try reducing the amount of lysis solution. Significant errors in weighing small amounts of tissue (<100 mg) can be introduced if the tissue is extremely wet. Tissue samples should be blotted dry just before weighing. Watery tissues may yield more RNA if they are lyophilized before disruption. This increases the ratio of lysis solution to dry-weight tissue.
What kind of Polyvinylpyrrolidone (PVP) do you recommend using with MagMAX Plant DNA and RNA isolation kits since there are different kinds available?

For the MagMAX Plant DNA Isolation Kit, we recommend using PVP M.W. 40,000, Cat. No. J62417.A1 (https://www.thermofisher.com/order/catalog/product/J62417.A1).

For the MagMAX Plant RNA Isolation Kit, we recommend using Plant RNA Isolation Aid, Cat. No. AM9690 (https://www.thermofisher.com/order/catalog/product/AM9690).

For Research Use Only. Not for use in diagnostic procedures.