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Invitrogen™ NorthernMax™-Gly Kit

Catalog No. AM1946
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AM1946 1 kit
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Catalog No. AM1946 Supplier Invitrogen™ Supplier No. AM1946
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Description

Includes

Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, Formaldehyde Load Dye, ULTRAhyb Buffer, 10X Denaturing Gel Buffer, and 10X MOPS Gel Running Buffer, Agarose-LE, Transfer Buffer, Low Stringency Wash Solution No. 1, High Stringency Wash Solution No. 2, and RnaseZap™, nuclease-free water

An alternative to formaldehyde-based systems for northern analysis and uses a glyoxal/DMSO Loading Solution for sample denaturation

  • Use of glyoxal/DMSO instead of formaldehyde for sample preparation avoids the need to pour and run gels in a fume hood, and eliminates the safety issues associated with use of formaldehyde
  • Glyoxal gels can be prepared at the lab bench: ;no noxious reagents
  • Increased sensitivity up to 100x over standard hybridization protocols
  • Reduce hybridization to 2 hours for many messages
  • Complete kit contains ULTRAhyb™ Hybridization Buffer and gel and wash reagents for 10 to 20 gels
  • Compatible with RNA, DNA, or oligonucleotide probes labeled isotopically or nonisotopically
  • Sufficient reagents are provided for processing 1000cm2 membrane
  • RNA samples denatured with glyoxal may show sharper bands on northern blots compared to samples denatured and run in the presence of formaldehyde
  • The streamlined NorthernMax-Gly procedure is more convenient and faster than the standard glyoxal gel protocol: A single reagent is used for sample denaturation and loading, the incubation time for sample denaturation has been reduced, and no recirculation of buffer is required during electrophoresis
  • The volume ratio of glyoxal denaturation solution to sample RNA is lower than many protocols, so that sample precipitation prior to gel loading is usually not required
  • An RNA sample and template for probe synthesis are also provided as controls for each kit
  • Compatible with glyoxal-containing gels

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage • Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, and Glyoxal Load Dye should be stored at -20°C.
• ULTRAhyb™ Buffer and 10X Gel Prep⁄Running Buffer should be stored at 4°C.
• Agarose-LE™, Transfer Buffer, Low Stringency Wash Solution #1, mL High Stringency Wash Solution #2, and RNaseZap™ should be stored at room temperature.
• Nuclease-free water may be stored at any temperature.
Gel Compatibility Glyoxal-Containing Gels
Quantity 1 kit
Shipping Condition Room Temperature
Product Type Northern Analysis Reagents

For Research Use Only. Not for use in diagnostic procedures.