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Invitrogen™ Ambion™ mRNA CATCHER™ PLUS Purification Kit DFS Item

LNA technology offers improved and faster performance

$489.30 - $1,902.60

Specifications

For Use With (Application) Microarray Analysis, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
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Includes:  1 96-well mRNA Catcher PLUS plate; 4mL 2X Lysis Buffer L20; 30mL Wash Buffer W15; 4mL Dilution Buffer DL1; 8mL Elution Buffer E3; 100μL 0.5M DTT (4°C)

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

K157003

DFS Item
 
invitrogen™
K157003
960 preps Each for $1,902.60

K157002

DFS Item
 
invitrogen™
K157002
96 preps Each for $489.30
Description & Specifications

Specifications

For Use With (Application) Microarray Analysis, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

• Automated protocols, high-throughput compatible
• Robust performance: high binding capacity per well (>80ng/well)
• Reproducible results: low CVs from well-to-well and plate-to-plate (∽ 2%)
• Optimized protocols: for small sample sizes (100 cells, 4mg tissue, and 40μL blood)
• Compatible plate design: may be used with most PCR cyclers

Automated Nucleic Acid Purification, Automated mRNA Isolation, DNA and RNA Purification and Analysis, RNA Extraction, mRNA Isolation

Shipping Condition: Wet ice