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Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene™ Blood RNA Tubes
Description
Includes
Kit Components Box 1: 1 PAXgene™ Resuspension Solution, 18mL; Wash Solution 1 Concentrate, 16mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle); Wash Solution 2 Concentrate, 2 x 30mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle); RNA Rebinding Buffer 10mL; Elution Buffer 10mL; Processing Plate (96-well) 1 plate; 2.0mL Tubes 100 tubes, RNA Binding Beads, 2mL. Box 2: 2 PAXgeneR Proteinase, 0.6mL; 10x TURBO™ DNase Buffer, 1mL; TURBO™ DNase, 1mL
Requires
Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g; 50mL conical tube adapters; 50mL conical tubes; Tabletop microcentrifuge or picocentrifuge (for For tube format users); Blood tubes; Magnetic stand
The MagMAX for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.
Maximize Total RNA Yields From Blood Samples
The MagMAX for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene Blood RNA Tubes. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 μg from a single PAXgene Blood RNA tube. A TURBO DNase step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene Blood RNA tubes as shown by TaqMan™ gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.
Rapid Processing Time
Processing times will vary based on format selected, but twelve PAXgene Blood RNA tubes can be processed manually with the MagMAX for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.
An Alternative to Full Purification
For users who perform primarily TaqMan-based real-time RT-PCR experiments, and do not need to archive RNA/cDNA, an alternative to the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene Blood RNA tubes without conventional RNA purification.
One-Stop Shopping
MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with reverse transcription mixes (SuperScript™), PCR master mixes (both TaqMan and SYBRbased), and other blood workflow products.
- Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
- Simple magnetic bead workflow—no spin columns
- Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors
- Suitable for use with automated protocols, high-throughput compatible
Flexible Purification Formats
- The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods
- For low-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5 mL microfuge tubes with the bead-RNA-complex remaining in a single processing tube until the final elution step
- For medium-to-high throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process
- For high-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing
- Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps
- The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX Express-96 Magnetic Particle Processor (deep well version only)
PAXgene™ and PreAnalytiX™ are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.
Specifications
Specifications
| Content And Storage | Box 1 |
| Isolation Technology | Magnetic Bead |
| Purification Time | Less than 2 hr. when using automated protocol |
| Elution Volume | 20 to 80 μL |
| Sample Type | Whole Blood |
| Final Product Type | Total RNA |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling |
| Quantity | 96 preps |
| Shipping Condition | Box 1: Room Temperature Box 2: Cold Gel |
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Frequently Asked Questions (FAQs)
Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.
Some bead carryover is common and has not been documented to affect downstream assays. The most common cause of bead carryover is too much input sample. The best way to determine if the sample amount is the cause of the issue is to run PBS with Xeno (artificial DNA/RNA) in the lysis buffer and then check if there is still bead carryover. If that is not the cause, the initial sample may require special processing, such as bead beating or liquification. If there is still bead carryover without the original sample, and in particular, if the carryover is present in the same wells for multiple runs, then this may be an instrument alignment issue.
Plastic consumables need to be purchased separately in order to use the MagMAX reagents on the MagMAX Express-96 or a KingFisher instrument. The particular consumables and the amount required will vary by kit and protocol so please check the user guide for the particular MagMAX kit to be sure that all required plastic consumables are available prior to sample processing.
Yes, reaction volumes for MagMAX kits can be scaled up. In general, the amount of MagMAX reagents should be scaled up proportionally for the increase in sample input amount. Please see the specific kit user guide for any recommendation. If none are included, then optimization may be necessary.
Addition of BME (2-mercaptoethanol) to the lysis buffer of MagMAX kits is fine. This can be beneficial with more RNase-rich samples like certain types of tissues.
In general, we recommend adding 0.3 µL BME per 100 µL of lysate.
For Research Use Only. Not for use in diagnostic procedures.