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Invitrogen™ MagMAX™-96 Blood RNA Isolation Kit
Description
This Ambion™ kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates. RNA purified using the kit is ideal for RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.
• Easily isolate viral or total RNA from whole blood
• Save precious time—complete the protocol in less than 1 hour
• Walk away—integrate with established robotic platforms
• Validated with human, bovine, porcine, and ovine whole blood
Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.
Isolate RNA from as Little as 50 μl of Blood:
For the highest-quality RNA, use whole blood samples that have not been frozen. Approximately 300–500 ng of intact RNA can be consistently purified from 50 μl of human whole blood. Bovine, porcine, and ovine whole blood samples can also be used. Yields vary from species to species (see data). The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood.
Isolate Viral and Total RNA from Whole Blood Samples:
The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination. The procedure is fast (less than 1 hour), simple, and well suited for automation. The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource. The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.
Specifications
Specifications
| Content And Storage | Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp. Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C. Store Lysis/Binding Enhancer and TURBO DNase at -20°C. |
| Format | 96-well Plate |
| Isolation Technology | Magnetic Bead |
| Sample Type | Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian) |
| Final Product Type | Total RNA, Viral RNA |
| High-throughput Compatibility | High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols |
| For Use With (Equipment) | MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting |
| No. of Reactions | 96 Preps |
| Prep Scale | < 100 μL Blood Samples (Small-scale) |
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Frequently Asked Questions (FAQs)
Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.
For Research Use Only. Not for use in diagnostic procedures.