missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. Invitrogen™ Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG

Invitrogen™ Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG

Catalog No. B40944
Encompass
Change view
Click to view available options
Quantity:
Sufficient for 150 Slides
Sufficient for 50 Slides
Product Type:
Tyramide Kit
Tyramide SuperBoost Kit
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Product Type
B40944 Sufficient for 50 Slides Tyramide SuperBoost Kit
B40925 Sufficient for 150 Slides Tyramide Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. B40944 Supplier Invitrogen™ Supplier No. B40944
Only null left

Description

Features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody. Alexa Fluor™ 488 Tyramide SuperBoost™ Kit combine brightness of AlexaFluor dyes with high signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce s

SuperBoost™ tyramide signal amplification is most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Features:

  • High sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
  • Simple protocol and detection using standard filters
  • Suitable for high-resolution multiplex images, co-label with DAPI, secondary antibodies, and other SuperBoost kits
  • Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Benefits:

  • Enhancement of signal using Alexa Fluor tyramides: Utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules
  • Poly-HRP enhancement: Several HRP enzymes are conjugated with short polymers, enhancing signal by several fold over regular HRP systems
  • Reaction stop solution: Include an HRP stop solution to halt the HRP reaction
  • Reduction of background: Include blockers for elimination or reduction of endogenous peroxidase and fluorescent background signals

Shelf Life:

  • Stable for 6 months after receiving

Specifications

Conjugate Alexa Fluor 594
Quantity Sufficient for 50 Slides
Content And Storage
  • 1 kit sufficient for 50 microscope slides (18 x 18 mm), containing:
Blocking buffer (1X), 22.5 mL
  • Poly-HRP-conjugated goat anti-rabbit secondary antibody (1X), 22.5 mL
  • Alexa Fluor tyramide reagent
  • Hydrogen peroxide
    		•
    Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
    Product Line SuperBoost
    Product Type Tyramide SuperBoost Kit

    Frequently Asked Questions (FAQs)

    With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?

    To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.

    Is it possible to perform dual TSA labeling with SuperBoost tyramide kits?

    Yes. This involves the sequential application of the antibodies and the tyramides with a HRP-quenching step between antibodies using H2O2.

    How are SuperBoost tyramide kits different from the original TSA labeling kits?

    The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.

    For Research Use Only. Not for use in diagnostic procedures.