missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. Invitrogen™ AIRE Monoclonal Antibody (5H12), eBioscience™, Invitrogen™

Invitrogen™ AIRE Monoclonal Antibody (5H12), eBioscience™, Invitrogen™

Catalog No. 14593437
Encompass
Change view
Click to view available options
Quantity:
2 mg
10 mg
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
14593437 2 mg
14593495 10 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. 14593437 Supplier Invitrogen™ Supplier No. 14593437
Only null left

Description

Rat Monoclonal Antibody

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2. Applications Reported: This 5H12 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of frozen tissue sections. Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression. A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes. It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP. Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD). AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity. Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Specifications

Antigen AIRE
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 5H12
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Aire
Gene Accession No. Q9Z0E3
Gene Alias Aire; AIRE protein; AIRE1; APECED; APECED protein; APECED protein homolog; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy); autoimmune regulator AIRE1a; LOW QUALITY PROTEIN: autoimmune regulator; OTTHUMP00000109529; PGA1
Gene Symbols Aire
Host Species Rat
Purification Method Affinity chromatography
Quantity 2 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 11634
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2c κ
Show More Show Less