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Gibco™ AAV-MAX Transfection Kit

Catalog No. A50516
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1 kit for 1 L of production
1 kit for 10 L of production
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A50516 1 kit for 10 L of production
A50515 1 kit for 1 L of production
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Catalog No. A50516 Supplier Gibco™ Supplier No. A50516

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.

Features of the AAV-MAX Transfection Kit include:

  • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
  • AAV-MAX booster—chemically defined component that works with the transfection reagent to achieve high transfection efficiency
  • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles
  • Robust transfection protocols are adaptable to large-scale transfection

Specifications

Cell Line HEK293
Classification Animal Origin-free
Content And Storage • 60 mL AAV-MAX Trasfection Reagent
• 30 mL AAV-MAX Transfection Booster
• 100 mL AAV-MAX Enhancer

Reagents should be stored at 2–8°C. Do not freeze.
Culture Type Suspension Cell Culture
Description Serum-free, animal origin-free, chemically defined
Cell Type Established Cell Lines
Format Kit
Sample Type Plasmid DNA
Transfection Technique Lipid-based Transfection, Transient Transfection
Expression System Mammalian
For Use With (Equipment) AAV-MAX System
For Use With (Application) AAV Production
Includes 100 mL Gibco AAV-MAX Enhancer, 60 mL Gibco AAV-MAX Transfection Reagent, 30 mL Gibco AAV-MAX Supplement
Packaging Type Bottle
Product Line AAV-MAX
Product Type AAV production transfection Kit
Quantity 1 kit for 10 L of production
Reagent Type Transfection kit
Shipping Condition Wet Ice
Sterility Sterile-filtered
Manufacturing Quality ISO 13485, ISO 9001 certified
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Frequently Asked Questions (FAQs)

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Enhancer and what does it do?

The AAV-MAX Enhancer increases AAV vector production in Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the AAV-MAX Enhancer are proprietary.
In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Transfection Booster and what does it do?

The AAV-MAX Transfection Booster enhances the efficiency of co-transfection of multiple plasmids into high-density Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the transfection booster are proprietary.
Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.
How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.
What is the optimal incubation time for the plasmid DNA/AAV-MAX Transfection Reagent/AAV-MAX Transfection Booster complex?

We recommend a complexation time of 20-30 mins. We recommend optimizing the complexation time for different sized plasmids.
Is it necessary to use the AAV-MAX Transfection Booster with the AAV-MAX Helper-Free AAV Production System? Will I see a drop in adeno-associated virus (AAV) titer if the booster is not used?

Yes, the AAV-MAX Transfection Booster facilitates transfection. If the booster is not used, it will reduce system performance.

For Research Use Only. Not for use in diagnostic procedures.