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Gibco™ AAV-MAX Transfection Kit

Catalog No. A50516
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1 kit for 1 L of production
1 kit for 10 L of production
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A50516 1 kit for 10 L of production
A50515 1 kit for 1 L of production
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Catalog No. A50516 Supplier Gibco™ Supplier No. A50516

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.

Features of the AAV-MAX Transfection Kit include:

  • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
  • AAV-MAX booster—chemically defined component that works with the transfection reagent to achieve high transfection efficiency
  • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles
  • Robust transfection protocols are adaptable to large-scale transfection

Specifications

Cell Line HEK293
Classification Animal Origin-free
Content And Storage • 60 mL AAV-MAX Trasfection Reagent
• 30 mL AAV-MAX Transfection Booster
• 100 mL AAV-MAX Enhancer

Reagents should be stored at 2–8°C. Do not freeze.
Culture Type Suspension Cell Culture
Description Serum-free, animal origin-free, chemically defined
Cell Type Established Cell Lines
Format Kit
Sample Type Plasmid DNA
Transfection Technique Lipid-based Transfection, Transient Transfection
Expression System Mammalian
For Use With (Equipment) AAV-MAX System
For Use With (Application) AAV Production
Includes 100 mL Gibco AAV-MAX Enhancer, 60 mL Gibco AAV-MAX Transfection Reagent, 30 mL Gibco AAV-MAX Supplement
Packaging Type Bottle
Product Line AAV-MAX
Product Type AAV production transfection Kit
Quantity 1 kit for 10 L of production
Reagent Type Transfection kit
Shipping Condition Wet Ice
Sterility Sterile-filtered
Manufacturing Quality ISO 13485, ISO 9001 certified
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For Research Use Only. Not for use in diagnostic procedures.