Gibco™ AAV-MAX Helper-Free AAV Production System Kit 

Description

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

• Components work synergistically to produce high AAV titers greater than 5 x 10¹⁰ vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection
• The components included in the AAV-MAX System Kit are:
  • 1 vial of Viral Production Cells 2.0
  • 1 L of Viral Production Medium
  • 1 AAV-MAX Transfection Kit for 1 L of production
  • 100 mL Viral-Plex Complexation Buffer
  • 100 mL AAV-MAX Lysis Buffer
  • 100 mL GlutaMAX Supplement

All components are also available for purchase separately.