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  7. 5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98+%, Thermo Scientific Chemicals

5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98+%, Thermo Scientific Chemicals

Catalog No. AAH5626503
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Catalog No. AAH5626503 Supplier Thermo Scientific Chemicals Supplier No. H5626503
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Description

It is an histochemical substrate for alkaline phosphatase. For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is an histochemical substrate for alkaline phosphatase. For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

Solubility
Soluble in water and toluene.

Notes
Moisture and light sensitive. Store in dry place. Keep away from oxidizing agents, heat and light.

Chemical Identifiers

CAS 102185-33-1
Molecular Formula C8H4BrClNNa2O4P
Molecular Weight (g/mol) 370.43
MDL Number MFCD00036757
InChI Key OAZUOCJOEUNDEK-UHFFFAOYSA-L
PubChem CID 6097197
IUPAC Name disodium;(5-bromo-4-chloro-1H-indol-3-yl) phosphate
SMILES [Na+].[Na+].[O-]P([O-])(=O)OC1=CNC2=CC=C(Br)C(Cl)=C12

Specifications

Quantity 1 g
Beilstein 4722243
Sensitivity Light and moisture sensitive
Synonym sodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 5-bromo-4-chloro-3-indolyl phosphate disodium salt, 5-bromo-4-chloro-3-indoxyl phosphate, disodium salt, bcip™, 1h-indol-3-ol, 5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, disodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 1h-indol-3-ol,5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, 5-bromo-4-chloro-3-indolyl phosphate, 5-bromo-4-chloro-3-indolylphosphate disodium salt
Solubility Information Soluble in water and toluene.
Formula Weight 370.43
Percent Purity ≥98%
Chemical Name or Material 5-Bromo-4-chloro-3-indolyl phosphate disodium salt

Safety and Handling

TSCA No
Recommended Storage Store at -20°C

RUO – Research Use Only