missing translation for 'onlineSavingsMsg'
Learn More
Learn More
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™
Commonly known as X-Gal. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals is a histochemical substrate for β-galactosidase. It is particularly useful in molecular biology, where β-galactosidase is frequently used as a report
$358.00 - $1138.44
Chemical Identifiers
| CAS | 7240-90-6 |
|---|---|
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight (g/mol) | 408.629 |
| InChI Key | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| Synonym | x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal |
| PubChem CID | 65181 |
| ChEBI | CHEBI:75055 |
| IUPAC Name | (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol |
| SMILES | C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|
ICN15000183
|
MP Biomedicals Inc
0215000183 |
250 mg |
Each for $358.00
|
|
|||||
|
ICN15000191
|
MP Biomedicals Inc
0215000191 |
1 g |
Each for $1,138.44
|
|
|||||
Description
5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques.
It is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
- Insertion of DNA into the lac Z region results in the loss of β-galactosidase activity
- Lac+ bacterial colonies resulting from a-complementation will appear blue
- Bacterial colonies containing plasmid with DNA inserted in the lac Z region will be incapable of a-complementation and will appear white
-
Chemical Identifiers
| 7240-90-6 | |
| 408.629 | |
| x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal | |
| CHEBI:75055 | |
| C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br |
| C14H15BrClNO6 | |
| OPIFSICVWOWJMJ-AEOCFKNESA-N | |
| 65181 | |
| (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol |
Specifications
| 7240-90-6 | |
| C14H15BrClNO6 | |
| x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal | |
| OPIFSICVWOWJMJ-AEOCFKNESA-N | |
| (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol | |
| 65181 | |
| 408.6 |
| 230°C (decomposition)(literature) | |
| 250 mg | |
| Soluble in DMF (2% w/v, clear and colorless solution). | |
| C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br | |
| 408.629 | |
| CHEBI:75055 | |
| Powder |
Safety and Handling
Recommended Storage : −20°C, desiccate, protect from light