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  6. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™

Catalog No. ICN15000191
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250 mg
1 g
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ICN15000191 1 g
ICN15000183 250 mg
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Catalog No. ICN15000191 Supplier MP Biomedicals Inc Supplier No. 0215000191
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Description

Commonly known as X-Gal. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals is a histochemical substrate for β-galactosidase. It is particularly useful in molecular biology, where β-galactosidase is frequently used as a report

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques.

It is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

  • Insertion of DNA into the lac Z region results in the loss of β-galactosidase activity
  • Lac+ bacterial colonies resulting from a-complementation will appear blue
  • Bacterial colonies containing plasmid with DNA inserted in the lac Z region will be incapable of a-complementation and will appear white

Chemical Identifiers

CAS 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight (g/mol) 408.629
InChI Key OPIFSICVWOWJMJ-AEOCFKNESA-N
Synonym x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal
PubChem CID 65181
ChEBI CHEBI:75055
IUPAC Name (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br

Specifications

Melting Point 230°C (decomposition)(literature)
Quantity 1 g
Solubility Information Soluble in DMF (2% w/v, clear and colorless solution).
Formula Weight 408.6
Physical Form Powder

Safety and Handling

Recommended Storage −20°C, desiccate, protect from light