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5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals™
Commonly known as X-Gal. 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside, MP Biomedicals is a histochemical substrate for β-galactosidase. It is particularly useful in molecular biology, where β-galactosidase is frequently used as a report
Supplier: MP Biomedicals Inc 0215000183
Description
5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques.
It is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
- Insertion of DNA into the lac Z region results in the loss of β-galactosidase activity
- Lac+ bacterial colonies resulting from a-complementation will appear blue
- Bacterial colonies containing plasmid with DNA inserted in the lac Z region will be incapable of a-complementation and will appear white
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Specifications
| 7240-90-6 | |
| C14H15BrClNO6 | |
| x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal | |
| OPIFSICVWOWJMJ-AEOCFKNESA-N | |
| (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol | |
| 65181 | |
| 408.6 |
| 230°C (decomposition)(literature) | |
| 250 mg | |
| Soluble in DMF (2% w/v, clear and colorless solution). | |
| C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br | |
| 408.629 | |
| CHEBI:75055 | |
| Powder |
Chemical Identifiers
| 7240-90-6 | |
| 408.629 | |
| x-gal, 5-bromo-4-chloro-3-beta-d-galactopyranosyloxy indole, 5-bromo-4-chloro-3-indolyl-beta-d-galactoside, unii-v595og374w, 5-bromo-4-chloro-3-indolyl beta-galactoside, 5-bromo-4-chloroindol-3-yl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactoside, 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside, 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside, indoxyl-gal | |
| CHEBI:75055 | |
| C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br |
| C14H15BrClNO6 | |
| OPIFSICVWOWJMJ-AEOCFKNESA-N | |
| 65181 | |
| (2S,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol |
Safety and Handling
Recommended Storage : −20°C, desiccate, protect from light