Invitrogen™ 4-HNE Competitive ELISA Kit

ELISA

The 4 Hydroxynonenal (4 HNE) ELISA quantitates 4 HNE in serum, plasma and other biological fluids. Principle of the method The Competitive multispecies ELISA research use only kit is designed to quantitatively measure The 4 Hydroxynonenal (4 HNE) independent of species. A 4 Hydroxynonenal standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to 4 Hydroxynonenal is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of 4 Hydroxynonenal in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.