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Invitrogen™ 25-Hydroxy Vitamin D3 Competitive ELISA Kit

Catalog No. EEL152
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96 Tests
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Catalog No. Quantity
EEL152 96 Tests
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Catalog No. EEL152 Supplier Invitrogen™ Supplier No. EEL152

Description

ELISA

25-OH Vitamin D3 (calcifediol) is a prohormone produced by the liver by hydroxylation of vitamin D3 and is used to measure vitamin D status and detect itamin D deficiency.

Specifications

Assay Range 3.13 to 200 ng/mL
Assay Sensitivity 1.88 ng/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target 25-OH Vitamin D3
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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