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2-Mercaptoethanol, Electrophoresis Grade, 98+%

Catalog No. AAJ6674230
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Catalog No. AAJ6674230 Supplier Thermo Scientific Chemicals Supplier No. J6674230
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CAS: 60-24-2 | C2H6OS | 78.13 g/mol

β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA. It is widely used for retarding oxidation of biological compounds in solution.

Solubility
Fully miscible in water. Soluble in alcohol, ether, benzene, and other organic solvents.

Notes
Handle under dry protective gas. Store in cool condition. Incompatible with air, moisture, oxidizing agents.

Chemical Identifiers

CAS 60-24-2
Molecular Formula C2H6OS
Molecular Weight (g/mol) 78.13
MDL Number MFCD00004890
InChI Key DGVVWUTYPXICAM-UHFFFAOYSA-N
Synonym 2-mercaptoethanol, mercaptoethanol, thioglycol, beta-mercaptoethanol, ethanol, 2-mercapto, 2-thioethanol, 2-hydroxy-1-ethanethiol, thioethylene glycol, 2-hydroxyethanethiol, 2-hydroxyethyl mercaptan
PubChem CID 1567
ChEBI CHEBI:41218
SMILES OCCS

Specifications

Melting Point -100°C
Color Colorless
Density 1.114 g/mL
Boiling Point 156°C to 158°C
Flash Point 73°C (163°F)
Odor Unpleasant
Linear Formula HSCH2CH2OH
Quantity 250 g
UN Number UN2966
Beilstein 773648
Sensitivity Air sensitive; Hygroscopic
Merck Index 14,5869
Solubility Information Fully miscible in water. Soluble in alcohol,ether,benzene,and other organic solvents.
IUPAC Name 2-sulfanylethanol
Formula Weight 78.13
Percent Purity ≥98%
Grade Electrophoresis
Physical Form Liquid
Chemical Name or Material 2-Mercaptoethanol
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Hazard Category H227-H301+H331-H310-H315-H317-H318-H373
Hazard Statement GHS H Statement
H301-H310-H318-H227-H315-H317-H335
Toxic if swallowed.
Fatal in contact with skin.
Causes serious eye damage.
Combustible liquid.
Causes skin irritation.
May cause an allergic skin reaction.
May cause respiratory irritation.
Precautionary Statement P210-P235-P260-P262-P264b-P270-P271-P272-P280-P301+P310-P302+P350-P304+P340-P305+P351+P338-P310-P311-P314-P330-P333+P313-P361-P363-P370+P378q-P501c
DOTInformation Transport Hazard Class: 6.1; Packaging Group: II; Proper Shipping Name: THIOGLYCOL
EINECSNumber 200-464-6
RTECSNumber KL5600000
TSCA Yes
Recommended Storage Ambient temperatures

RUO – Research Use Only