MilliporeSigma Western Blotting Solutions

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Designed to Help You Work More Efficiently

Get consistent and accurate results with a comprehensive range of MilliporeSigma products for western blotting:

  • Immobilon GO, the new walk-away immunodetection device
  • The SNAP I.d.™ System, a fast detection platform
  • Immobilon™-P membrane, the most referenced PVDF membrane on the market 
  • Immobilon-E, the first prewet PVDF membrane without alcohol
  • Plus detection reagents that are simple to use

Immobilon NOW Transfer Membrane Video

See how to get perfectly sized western blotting membrane every time with Immobilon NOW Transfer Membranes.

Request a Protein Blotting Handbook

Get valuable tips and advice to help improve your western blotting from this handbook. Just fill out the form to request a copy today.

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Immobilion-E Blotting-Sandwiches-18-2865

Immobilon™-E Transfer Membrane

Available in a roll or easy-to-use precut sheets that fit standard mini or midi blots, these membranes eliminate the need for alcohol pre-wetting.

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Immobilon-E Blotting Sandwiches

A convenient way to process multiple Western blots for standard tank transfers. Each sandwich has Immobilon-E membranes interleaved with sheets of blotting paper.

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Immobilon-NOW Transfer Membranes

Compatible with mini or midi gels, these membranes offer the convenience of pre-cut sheets with the flexibility of membrane rolls.

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Immobilon Transfer Membrane Half Rolls

Save money and custom-cut your Immobilon-E, -FL, -P, or ‑PSQ transfer membranes from these convenient half rolls.

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Immobilon Signal Enhancer for Immunodetection

Combines signal amplification and blocking in a ready-to-use reagent that amplifies low signal intensity without amplifying the noise.

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Immobilon Block Noise Canceling Reagents

Minimize background noise for easier detection of even low-abundance proteins with these protein-free blocking reagents.

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ReBlot-Antibody-Stripping-Solution-18-2865-

ReBlot™ Antibody Stripping Solution

Now you can re-use your western blots ─ strip just the antibodies from blots at room temperature in approximately 15 minutes.

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Immobilon-Western-Chemiluminescent-HRP-Substrate-18-2865-

Immobilon Western Chemiluminescent HRP Substrate

Combining high sensitivity and low background, this substrate provides high sensitivity over a broad detection range.

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Immobilon ECL Ultra Western HRP Substrate

This ultra-sensitive reagent has a long signal duration for detecting horseradish peroxidase (HRP)-labeled antibodies on Western blots.

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Immobilon Classico Western HRP Substrate

A premixed, ready-to-use reagent for the chemiluminescent detection of high- to medium-abundance proteins in western blots that use HRP-conjugated antibodies.

100mL  500mL


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Immobilon Crescendo Western HRP Substrate

A premixed, ready-to-use reagent for the chemiluminescent detection of medium- to low-abundance proteins in western blots that use HRP-conjugated antibodies.

100mL   500mL

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Immobilon Forte Western HRP Substrate

Detect medium- to low-abundance proteins in western blots that use HRP-conjugated antibodies with this premixed detection reagent that’s stable at room temperature and more sensitive.

100mL   500mL


SNAP-2.0-Protein-Detection-System-18-2865

SNAP i.d.™ 2.0 Protein Detection System

Uses a vacuum to actively drive antibodies and buffers right through the membrane to shorten the time for blocking, washing, and antibody incubations to just 30 minutes.

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Immobilon GO Immunodetection Device

Immobilon GO Immunodetection Device

Apply the principles of lateral flow assays to western blots for walk-away processing in three easy steps and results in as little as three hours.

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Immobilon-P PVDF Transfer Membranes

Immobilon-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices with superior handling, staining, and resistance.

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