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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 20 Peptide

Catalog No. PV4532
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800 x 20 μL Assays
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PV4532 800 x 20 μL Assays
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Catalog No. PV4532 Supplier Thermo Scientific™ Supplier No. PV4532
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a synthetic peptide substrate for NDR1.

Specifications

Product Line Z ́-LYTE
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage Z ́-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Excitation/Emission 405/465, 535
Format 384-well Plate
For Use With (Application) FRET
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Quantity 800 x 20 μL Assays
Readout End Point
References EMBO J. vol. 17, pp. 5913-22 (1998)
Shipping Condition Dry Ice
Source Based on Synthetic Peptide Substrate for NDR1 (KKRNRRLSVA)
Substrate Properties Peptide-Based Substrate
Target Entry PAK4, RPS6KA6, PAK7, PAK6, PAK2, PAK3
Target Enzyme Serine/Threonine Kinases
Validated Kinases PAK2, PAK3, PAK4, PAK6, PAK7, RPS6KA6
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For Research Use Only. Not for use in diagnostic procedures.