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  7. Gibco™ William's E Medium, no glutamine

Gibco™ William's E Medium, no glutamine

Numéro de catalogue. 12551032
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500 mL
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Numéro de catalogue. Quantity
12551032 500 mL
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Numéro de catalogue. 12551032 Fournisseur Gibco™ Code fournisseur 12551032
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Description

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells.

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

This William's E Medium is modified as follows:

  • With: Phenol Red
  • Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Dual-Site cGMP Manufacturing

Gibco™ William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Spécifications

Form Liquid
Product Type William's E Medium
With Additives Phenol Red, Sodium Pyruvate
Without Additives No Glutamine, No HEPES
For Use With (Application) Hepatocytes and hepatic models, mammalian cell culture, pharma and biopharma, primary cell culture
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Type Mammalian Cell Culture