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TRIS-buffered saline (TBS, 10X), with 1% Triton™ X-100, Thermo Scientific™

Catalog No. AAJ62533AP
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AAJ62533AP 500mL
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Catalog No. AAJ62533AP Supplier Thermo Scientific Chemicals Supplier No. J62533AP

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Applications
It used in some biochemical techniques that is maintain the pH within a relatively narrow range. Tris-HCl is commonly used to make TBS buffers. Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash micro titer plates for western blot experiments and screening methods. it is suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

Solubility
Fully miscible in water.

Notes
250mM Tris, 27mM KCl, 1.37M NaCl, pH 7.4

Chemical Identifiers

CAS 77-86-1
Molecular Formula C4H11NO3
Molecular Weight (g/mol) 121.136
MDL Number MFCD00132476
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
PubChem CID 6503
ChEBI CHEBI:9754
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
SMILES C(C(CO)(CO)N)O

Specifications

Concentration 10X
Quantity 500mL
Solubility Information Fully miscible in water.
Chemical Name or Material TRIS-buffered saline, with 1% Triton™ X-100
Hazard Category H319
Precautionary Statement P264b-P280i-P305+P351+P338
TSCA Yes
Recommended Storage Ambient temperatures

RUO – Research Use Only