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Thermo Scientific™ TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20

Catalog No. AAJ60448AP
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AAJ60448AP 500mL
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Catalog No. AAJ60448AP Supplier Thermo Scientific™ Supplier No. J60448AP

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Applications
TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein:protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate. TBS-Tween 20 is often used for washing steps.

Solubility
It is soluble in water.

Notes
250mM Tris, 27mM KCl, 1.37M NaCl, pH 7.4 with 0.5% Tween 20

Specifications

CAS 77-86-1
Concentration 10X
Quantity 500mL
Molecular Formula C4H11NO3
MDL Number MFCD00678198
Solubility Information It is soluble in water.
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
SMILES C(C(CO)(CO)N)O
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
Molecular Weight (g/mol) 121.136
PubChem CID 6503
ChEBI CHEBI:9754
Chemical Name or Material TRIS-buffered saline, with 0.5% Tween 20
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Safety and Handling

TSCA Yes
Recommended Storage Ambient temperatures

RUO – Research Use Only