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Thermo Scientific™ TR-FRET Coregulator Buffer B

Catalog No. PV4418
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25 mL
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PV4418 25 mL
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Catalog No. PV4418 Supplier Thermo Scientific™ Supplier No. PV4418

Description

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

Specifications

For Use With (Equipment) LanthaScreen™ Nuclear Receptor Cofactor Interaction Assays
Solubility Water soluble
For Use With (Application) Pharma and biopharma, target and lead identification and validation, drug discovery and development, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
Description TR-FRET Coregulator Buffer B, 25mL, Liquid, Store at 20 to 30°C, Water Soluble, Used For Pharma & Biopharma, Target & Lead Identification & Validation
Content And Storage TR-FRET Coregulator Buffer B may be shipped frozen or at room temperature.Store at room temperature (20°C to 30°C).
Quantity 25 mL
Form Liquid
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice

Frequently Asked Questions (FAQs)

I have no assay window in my TR-FRET assay. What is the reason?

The single most common reason that a TR-FRET assay fails is that an incorrect choice of emission filters was made. Unlike other fluorescent assays, the filters used in a TR-FRET assay must be exactly those that are recommended for your instrument as the emission filter choice can make or break the assay. The excitation filter has more of an impact on the assay window. Please refer to our instrument setup guides in our instrument compatibility portal (https://www.thermofisher.com/instrumentsetup). If your instrument is not listed there, please contact Drug Discovery Technical Support at drugdiscoverytech@thermofisher.com.

Note: Test your microplate reader's TR-FRET setup before you begin any work with your assay. Using reagents that you have already purchased for your assay, you can test the microplate reader's TR-FRET setup. Please refer to the Terbium (Tb) Assay (http://tools.thermofisher.com/content/sfs/manuals/LanthaScreen_Tb_Instrument_Control_Application_Note17Sep10.pdf) and Europium (Eu) Assay (http://tools.thermofisher.com/content/sfs/manuals/Eu_Instrument_Control_AppNote_12Nov10.pdf) Application Notes for plate reader setup.

For Research Use Only. Not for use in diagnostic procedures.