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Cytiva Thermo Sequenase DNA Polymerase

Catalog No. p-7087576
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09928126 1,000 Units
09928127 10,000 Units
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Catalog No. 09928126 Supplier Cytiva Supplier No. E79000Y
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Description

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
  • ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
  • Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
  • An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
  • Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

Specifications

Content And Storage -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
Description Thermo Sequenase DNA Polymerase
Quantity 1,000 Units
For Use With (Application) Sequencing, Genotyping
No. of Reactions 32 units/μL
Includes Thermo Sequenase with Thermoplasma Acidophilum Inorganic Pyrophosphatase (TAP), Dilution Buffer, and Reaction Buffer
Source E. coli clones that overproduce the protein