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Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

$1,350.00 - $8,773.00

Specifications

For Use With (Application) DNA Sequencing, and SNP Genotyping Applications
Storage Requirements -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
Includes Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer
Source E. coli clones that overproduce the protein
No. of Reactions 32 units/µL
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
09928126
View Documents Promotion Details
Cytiva
E79000Y
1,000 Units
Each for $1,350.00
 
09928127
View Documents Promotion Details
Cytiva
E79000Z
10,000 Units
Each for $8,773.00
 
Description

Description

  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
  • ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
  • Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
  • An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
  • Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Specifications

Specifications

DNA Sequencing, and SNP Genotyping Applications
Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer
32 units/µL
-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
E. coli clones that overproduce the protein
SDS
Product Certifications