Cytiva Thermo Sequenase DNA Polymerase

Numéro de catalogue. 09928126

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1,000 Units

10,000 Units

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Numéro de catalogue.	Quantity
09928126	1,000 Units
09928127	10,000 Units

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Numéro de catalogue. 09928126 Fournisseur Cytiva Code fournisseur E79000Y

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Description

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

Uniform and easy-to-read band patterns
Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

Content And Storage	-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
Description	Thermo Sequenase DNA Polymerase
Quantity	1,000 Units
For Use With (Application)	Sequencing, Genotyping
No. of Reactions	32 units/μL
Includes	Thermo Sequenase with Thermoplasma Acidophilum Inorganic Pyrophosphatase (TAP), Dilution Buffer, and Reaction Buffer
Source	E. coli clones that overproduce the protein