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Thermo Scientific™ EpiJET™ Bisulfite Conversion Kit

The EpiJET Bisulfite Conversion Kit is used for an efficient bisulfite modification of DNA for methylation analysis.

Manufacturer:  Thermo Scientific™ K1461

Catalog No. FERK1461



Description

Description

The EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR.
The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing.
Highlights

  • High conversion efficiency and specificity (≥99%)
  • Flexibility, provided by short DNA conversion protocol
  • Pure DNA, suitable for downstream applications and long-term storage
Applications
Converted DNA can be used in:
  • PCR
  • qPCR
  • Combined Bisulfite Restriction Analysis (COBRA)
  • Sequencing
Includes
  • Modification Reagent
  • Modification Solution I
  • Modification Solution II
  • Binding Buffer for Bisulfite Conversion Kit
  • Wash buffer (conc.) for Bisulfite Conversion Kit
  • Desulfonation Buffer (conc.)
  • Elution Buffer
  • DNA Purification Micro Columns & Collection Tubes

Hazardous: Yes
Quality Control: The kit is functionally tested by analyzing bisulfite converted fully methylated (by M.SssI) Human Genomic DNA. Performance of the kit is assayed by qPCR with primers, specific for converted and unconverted DNA. The kit passes QC requirements for DNA recovery and conversion efficiency.
Shelf Life: 24 Months
Shipping Condition: Refrigerated Gel Packs
Storage Condition: Ambient

Specifications

Specifications

Five vials Modification Reagent, Modification Solution I (1.2mL), Modification Solution II (0.35mL), Binding Buffer for Bisulfite Conversion Kit (25mL), Wash buffer (conc.) for Bisulfite Conversion Kit (9mL), conc. Desulfonation Buffer (3.5mL), Elution Buffer (1.5mL), DNA Purification Micro Columns and Collection Tubes (50)
50 reactions
Converted DNA can be used in: PCR, qPCR, Combined Bisulfite Restriction Analysis (COBRA), Sequencing
Store all components of the kit tightly capped at room temperature (15deg. to 25deg.C). Modification Reagent is light sensitive, therefore minimize its exposure to light. Prepared Modification Reagent solution can be stored for up to one week at 4deg.C or 4 weeks in the dark at -20deg.C.
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