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Thermo Scientific™ dsDNase

Catalog No. FEREN0771
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FEREN0771 50 reactions
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Catalog No. FEREN0771 Supplier Thermo Scientific™ Supplier No. EN0771
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Description

dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.

Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA such as cDNA and primers are not cleaved. dsDNase is easily inactivated by modetate heat treatment (55°C). These features make dsDNase an excellent choice for gDNA elimation prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

Highlights

  • Highly specific degradation of double-stranded DNA
  • Fast 2 min protocol for gDNA removal prior reverse transcription
  • Heat-labile—irreversibly inactivated by moderate heat treatment (55°C)

Applications

  • Genomic DNA removal from RNA samples prior first strand cDNA synthesis, RT-PCR and RT-qPCR

Specificities of dsDNase—dsDNase activity towards double- and single-stranded DNA and RNA oligonucleotides was measured. Relative activity values indicate that dsDNase is highly specific to double-stranded DNA.

  • dsDNA - 100% relative activity
  • ssDNA - <0.03% relative activity
  • dsRNA - <0.01% relative activity
  • ssRNA - <0.01% relative activity

Specifications

Description DNase
Quantity 50 reactions

Frequently Asked Questions (FAQs)

Does dsDNase cleave ssDNA?

No, dsDNase cleaves only double-stranded DNA. However, if ssDNA forms double-stranded structures, it will act as a target for dsDNase. Because of this, long and complex ssDNA may be partially degraded. Therefore we recommend additional dsDNase inactivation step for RT-PCR amplification of targets 3 kb or longer. The inactivation step should be performed by 5 min incubation at 55 degrees C in the presence of 10 mM DTT.
Can dsDNase be added directly to a reverse transcription reaction to remove genomic DNA?

No. RT reaction composition inhibits dsDNase activity, and genomic DNA removal may be incomplete.
When using the Maxima or Maxima H Minus First Strand cDNA Synthesis kits with dsDNase, can the genomic DNA elimination step be omitted?

Yes. dsDNase buffer is not needed for subsequent reverse transcription reaction. If RNA sample is not contaminated with gDNA, dsDNase treatment may be omitted.

For Research Use Only. Not for use in diagnostic procedures.