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Gibco™ Tetracycline Hydrochloride

Catalog No. A39246
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500 mg
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A39246 500 mg
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Catalog No. A39246 Supplier Gibco™ Supplier No. A39246
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Description

Tetracycline is a yellow, odorless, crystalline powder used as an agent to activate gene expression from an inducible expression plasmid in engineered cells expressing the tetracycline repressor protein

Tetracycline Hydrochloride is the hydrochloride salt of tetracycline

Specifications

Type Tetracycline Hydrochloride
Physical Form Powder
Quantity 500 mg
Shipping Condition Wet Ice

Frequently Asked Questions (FAQs)

Can I use doxycycline instead of tetracycline as an inducer in the Expi293 Inducible Expression System?

Doxycycline may be used as an alternative inducing agent in the Expi293 Inducible System. It is similar to tetracycline in its mechanism of action. Doxycycline has been shown to have a longer half-life than tetracycline (48 hr vs. 24 hr, respectively). If the use of doxycycline is desired with the Expi293 Inducible System, we recommend performing a dose-response curve to determine the optimal concentration for induction.

How much tetracycline do I need to induce expression in Expi293F Inducible or Expi293F Inducible GnTI- cells?

We recommend starting with a tetracycline concentration of 1 µg/mL to induce the expression of your protein of interest. However, you can modulate the level of expression by treating cells with different amounts of tetracycline. We recommend performing a dose-response curve (0-1 µg/mL; 2-fold dilution) to determine the optimal concentration of tetracycline required to induce your protein of interest.

For Research Use Only. Not for use in diagnostic procedures.