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Invitrogen™ SureLock™ Tandem Midi Blot Module
Description
The SureLock Tandem Midi Blot Module is compatible with all Invitrogen midi gels (blot size: 8.5 cm x 14 cm). The blot module accommodates one midi gel per wet transfer. The unique design make the module easy to use, while the efficient size of the inner core uses less transfer buffer per transfer (only ∼300 mL per transfer). Since transfer buffer contains methanol, this also results in less hazardous waste requiring special disposal. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes in 30 minutes and at room temperature.
Features:
- Integrative design—used with the SureLock Tandem Midi Gel Tank, eliminates the need for a separate transfer tank
- Efficient size—transfer buffer needed is about one-third of traditional wet-transfer systems reducing methanol/transfer buffer waste and cost
- Quick set-up (time & materials)
- Compatible—efficiently transfers all Invitrogen pre-cast midi gels
- Smart design—anode and cathode halves are hinged and connected by a slider. The design enables all parts to be permanently connected eliminating the need for multiple accessories.
- Low voltage transfer (25 volts)—highly conductive anode plate separated by only 0.5 inches from the cathode plate enables efficient transfer at 25 volts within 30 minutes
- Room temperature transfer—eliminates the need to pre-chill buffers overnight and the hassle and messiness of ice baths used with traditional wet-transfer systems that require 100 volts for transfer and generate excessive heat
- Fast and efficient—30 minute transfer without compromising performance
- Simple clean up—empty, rinse, and dry (no spills or ice disposal)
- Optional SureLock Tandem Transfer Tray — can be used to set up wet transfer of midi gels using SureLock Tandem Blot Module
Specifications
Specifications
| Content And Storage | Contains: 1 SureLock Tandem Midi Blot Module • 1 Blotting Tweezer • 1 Blotting Roller • 4 Blotting Sponges |
| For Use With (Application) | Wet Transfer |
| For Use With (Equipment) | SureLock™ Tandem Midi Gel Tank |
| Gel Compatibility | Midi Precast Protein Gels |
| Gel Size | Midi (8 x 13 cm) |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Mode of Transfer | Wet |
| Product Line | SureLock |
| Type | Blot Module |
| For Use With | SureLock Tandem Midi Gel Tank (STM1001) |
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Frequently Asked Questions (FAQs)
Unfortunately, the PowerEase Touch 120W power supply has a maximum output of 500 mA and is not suitable for transferring two midi gels. For wet transfer, we recommend using the PowerEase Touch Power Supply 350W or 600W unit, which can deliver up to 3 A. More information about these power supply units can be found on our website.
No, most transfer applications require high current and therefore, we recommend using a high current power supply like PowerEase Touch 350W Power Supply.
We offer the following pre-cut membranes and filters for the SureLock Tandem Midi Blot Module:
- SureLock Tandem Midi Pre-cut Membranes and Filters, 0.45 µm, nitrocellulose, Cat. No. STM2008
- SureLock Tandem Midi Pre-cut Membranes and Filters, 0.2 µm, nitrocellulose, Cat. No. STM2007
- SureLock Tandem Midi Pre-cut Membranes and Filters, 0.45 µm, PVDF, Cat. No. STM2006
The SureLock Tandem Midi Blot Module (Cat. No. STM2001) is compatible with the SureLock Tandem Midi Gel Tank.
Yes, the following replacement parts are available for the SureLock Tandem Midi Blot Module:
- SureLock Tandem Midi Gel Tank Replacement Tank, Cat. No. STM1003
- SureLock Tandem Midi Gel Tank Replacement Lid, Cat. No. STM1002
- SureLock Tandem Midi Gel Tank Replacement Cassette Clamp, Cat. No. STM1004
- Gel Knife, Cat. No. EI9010
- SureLock Tandem Midi Sponge Kit, Cat. No. STM2002
- Blotting Roller, Cat. No. LC2100
- SureLock Tandem Midi Blot Module Replacement Gasket Kit , Cat. No. STM2003
- SureLock Tandem Transfer Tray, Cat. No. STM3001