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  6. MilliporeSigma™ Stem Cell Qualified ECM Gel

MilliporeSigma™ Stem Cell Qualified ECM Gel

Catalog No. CC1315ML10P
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5 mL
10 x 5 mL
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CC1315ML10P 10 x 5 mL
CC1315ML 5 mL
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Description

Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.

  • Stem Cell Qualified ECM Gel is a soluble basement membrane extract (BME) purified from Engelbreth-Holm-Swarm (EHS) tumor that has been pre-qualified for human ES/iPS cell culture
  • The matrix polymerizes at 20 to 40°C to form a reconstituted basement membrane
  • ECM Gel contains laminin, collagen IV, entactin and heparan sulfate among other ECMs
  • The matrix has been extensively tested and optimized for human ES/iPS cell culture
  • Eliminates the need for time-consuming prescreening and lot identification
  • Provides an optimized ECM coating necessary for long term culture of pluripotent human ES/iPS cells
  • Supports feeder-free culture of human ES/iPS cells in serum-free media
  • Compatible with all feeder free human ES/iPS cell expansion media (i.e. mTeSR™, PluriSTEM™)

Specifications

Form Gel
For Use With (Application) Human ES and iPS cell culture
Quantity 10 x 5 mL
Assays < 5% spontaneous differentiation for 3 passages using Human iPS cells
Endotoxin Level < 20 EU/mL
Color Red, clear of particulates
Content And Storage Store at -20°C, thaw overnight on ice in a 2 to 8°C refrigerator
Description Basement membrane extract (BME)