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Thermo Scientific™ Spectra™ Multicolor Low Range Protein Ladder

Catalog No. pi26628
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PI26628 250 μL
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Catalog No. PI26628 Supplier Thermo Scientific™ Supplier No. 26628
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Description

A mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ Spectra Multicolor Low Range Protein Ladder is a mixture of six (6) proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Highlights:

  • Size range – six proteins and peptides spanning 1.7 to 40kDa

  • Multicolor – three different colors for unambiguous band-size assignment

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel

Specifications

Content And Storage Contents: one vial of 250 μL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C
Detection Method Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Number of Markers 6
Ready to Load Yes
Recommended Applications Tricine gels
Size Range 1.7 to 40 kDa
For Use With (Application) Tricine gels
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 40, 25, 15, 10, 4.6, 1.7 kDa
Quantity 250 μL
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Line Spectra
Product Type Protein Ladder
Stain Type Blue, Orange, Green
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.