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Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder

A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

Manufacturer:  Thermo Scientific™ 26625

Catalog No. PI26625



Description

Description

Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Highlights:

  • Size range – eight proteins spanning 40 to 300kDa

  • Multicolor – three different colors for unambiguous band-size assignment

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel

Specifications

Specifications

colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel)
Spectra™
40 to 300 kDa
300, 250, 180, 130, 100, 70, 50, 40 kDa
2 x 250 mL
8
Tris-acetate gels
SDS-PAGE
3 colors: Blue, Orange, Green
Contents: two vials of 250 µL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

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