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Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits

Catalog No. 13622459
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Description:
Magnetic
Magnetic with SOLAμ SPE and collection plate
Non-magnetic
Non-magnetic with SOLAμ SPE and Collection Plate
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Catalog No. Description
13622459 Magnetic
13622456 Non-magnetic
13622457 Non-magnetic with SOLAμ SPE and Collection Plate
13622458 Magnetic with SOLAμ SPE and collection plate
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Catalog No. 13622459 Supplier Thermo Scientific™ Supplier No. 60110104
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Description

Obtain fast, simple, highly reproducible, and sensitive streptavidin immunocapture and trypsin protein digestion in a single well for your biomarker studies.

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The kit purifies various target biomolecules via the formation of strong streptavidin-biotin bond.

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

  • Fast
  • Simple
  • Highly reproducible
  • Sensitive
Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.

Specifications

Description Magnetic
Capture Streptavidin
Cleavage C-terminal side of lysine and arginine amino acids
Enzyme Trypsin
Format SMART Digest Resin, Magnetic
For Use With (Application) Peptide Quantitation
Product Line SMART Digest
Type Immunocapture + Protein Digestion combined on a single bead
Includes SMART Digest IA Kit, Av magnetic