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Invitrogen™ SlowFade™ Glass Soft-set Antifade Mountant, with DAPI
Description
It is formulated to have a refractive index of 1.52 for sharper images and antifade components to resist photobleaching. The result is sharp, bright images with almost any fluorophore, with wide-field, confocal, or super-resolution microscopes, at focal depths of 0–500 μm.
Since SlowFade Glass Antifade Mountant is non-curing, cells can be imaged immediately after mounting, without any of the adverse effects on cell morphology that a curing mountant might cause. It comes ready to use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.
Features of SlowFade Glass Antifade Mountant include:
• Ready-to-use, glycerol-based, non-curing antifade mountant
• Refractive index of 1.52, with minimized spherical aberrations
• Up to three times better axial resolution compared to regular mountant
• Visible tissue optical clearing, even in sections up to 1-mm thick
• Sharp, bright images at any focal depth from 0 to 500 μm
Sharp images for deep 3D imaging
SlowFade Glass Antifade Mountant features a refractive index of 1.52 (similar to glass coverslips), with superior photobleach protection. It is compatible with immersion oil and oil-immersion microscope optics, enabling a refraction-free light path and minimized spherical aberration, which improves axial resolution by three times (at 150-μm focal depth) as measured by point spread function. When 1-mm thick tissue sections are mounted, there is visible optical tissue clearing, enabling deep 3D imaging.
Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, Slowfade Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes, and fluorescent proteins, including GFP, RFP, and mCherry. SlowFade Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.
Non-curing mountant which does not expand or shrink specimens
Most tissue clearing techniques either expand or shrink the specimens, causing artifacts in specimen morphology. SlowFade Glass Antifade Mountant is non-curing and does not cause expansion or contraction of specimens, which we have verified using Hela spheroids and rat brain sections. This characteristic allows 3D imaging of truly native specimen morphology. The specimens are available for imaging immediately after mounting, and properly sealed slides can be stored for weeks or months for excellent
Specifications
Specifications
| Quantity | 1 x 2 mL |
| Product Type | Antifade Mountant |
| Content And Storage | Contains 1 dropper bottle containing 2 mL reagent |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Product Line | SlowFade |
| Reagent Type | Antifade Solution |
| Volume (Metric) | 2 mL |
Frequently Asked Questions (FAQs)
We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.
When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.
When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.
No. SlowFade Glass Soft-set Antifade Mountant with DAPI (Cat. No. S36920) does not maintain the structures of fluorescent proteins over time. The proteins will naturally unfold, and this unfolding would result in the loss of fluorescence.
To remove any tiny air bubbles, place the vial of SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), within a vacuum chamber with the vial cap loosened. Degas the solution by applying vacuum through either a T-connector on the faucet or a very brief application of vacuum from a vacuum pump.
No. SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), is an aqueous-based mountant.
No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.
For Research Use Only. Not for use in diagnostic procedures.