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Ambion™ Silencer™ Validated siRNA

Catalog No. AM51342
Encompass
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Purity:
In Vivo
Quantity:
1 μmol
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Catalog No. Purity Quantity
AM51342 In Vivo 1 μmol
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Catalog No. AM51342 Supplier Ambion™ Supplier No. AM51342
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Description

Silencer™ Select siRNAs are classic 21-mers which incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry.

  • The Silencer™ Select siRNAs are classic 21-mers which incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry
  • Silencer™ Select Validated siRNAs are functionally tested to reduce target gene expression
  • Sets of Silencer™ Select siRNA Libraries are also available in tubes or plate for high throughput screening
  • The Silencer™ Select Positive and Negative Control RNAs should be included in every siRNA experiment

The benefits of using Silencer™ Select siRNAs include:

  • Potent – up to 100-fold more potent than currently available siRNAs and fewer off-target effects
  • Most Specific – LNA™ chemical modifications reduce off-target effects by up to 90%
  • Reliable – demonstrated improvements in consistency and reliability of phenotypic results
  • Guaranteed – 100% guaranteed to silence, the best guarantee in the industry

Note: Silencer™ Pre-Designed & Validated siRNAs are designed using our first-generation algorithm and DO NOT include any chemical modifications. They are recommended for completing in vitro RNAi studies that were started using Silencer™ siRNAs. For new studies we recommend Silencer Select siRNAs.

Approximate Turnaround Times*

  • Standard purification: 4 business days
  • HPLC purification: 6 business days (20 and 40 nmol); 15 business days (250 nmol)
  • In Vivo Ready: 15 business days (250 nmol)

Specifications

Content And Storage Store at room temperature
Description Silencer™ Validated siRNA
RNAi Type siRNA
Product Line Silencer
Purity In Vivo
Quantity 1 μmol
Temperature (Metric) Store at room temperature

Frequently Asked Questions (FAQs)

Where should I add a fluorescent tag to minimize any negative functional effect on my siRNA?

For any RNAi duplex (miRNA or siRNA) there is a possibility that adding a modification will affect how the antisense strand interacts with RISC and aligns with its mRNA target, thus impacting functionality. For that reason, we recommend that you label the 5' end of the sense strand for siRNA (or passenger strand for miRNA). Since the 5' end of the antisense (guide strand) is what gets recognized by RISC, it is best to add the fluorescent modification to the end of the duplex on the strand that does not interact with RISC. You can also test a labeled vs. non-labeled RNAi duplex to determine if the modification is affecting the siRNA or miRNA functionality.

How can I find the GC content for the predesigned Silencer siRNA or Silencer Select siRNA sequences prior to purchase?

Please contact Technical Support at techsupport@thermofisher.com to request the GC content for one of our predesigned Silencer siRNA or Silencer Select siRNA sequences.

For Research Use Only. Not for use in diagnostic procedures.