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SGKL (SGK3) Recombinant Human Protein DFS Item

SGKL (SGK3) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3859

Catalog No. PV3859



Description

Description

SGKL (SGK3) is a serine⁄threonine protein kinase which is similar to serum- and glucocorticoid-induced protein kinase (SGK). Unlike SGK, SGKL is not induced by serum or glucocorticoids. It is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK.
Specifications

Specifications

NP_037389, S487D
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
74.3kDa
80% by SDS-PAGE
–80°C
1 Each
SGKL (SGK3)
SGK3 (SGKL) was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 50μM crosstide peptide substrate (GRPRTSSFAEG) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
SGK
SGKL (SGK3) Recombinant Human Protein
Human
0.14mg/mL
1385, 23678
SGKL (SGK3) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
SGKL (SGK3) Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 87-496
240nmoles of phosphate transferred to Crosstide peptide substrate (GRPRTSSFAEG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.